Abstract Rats INTRODUCTION Bone regeneration and healing of defects


Objectives: The healing of
bone defects in the craniofacial region is an important clinical issue. We aimed
to compare the effects of octacalcium phosphate (OCP) and the combination of OCP/gelatin
(OCP/Gel) on calvarial bone regeneration in rats.

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Materials and Methods: In this study, 72 male Sprague Dawley rats were randomly assigned to the OCP
(n=24), OCP/Gel (n=24), and control groups (n=24). Lesions with a diameter of 9
mm were created in the parietal bone and were filled with 9-mg OCP and OCP/Gel
disks. In the control group, no substance was implanted in the defect. Sampling
was performed on days 10, 14, 21, and 28 after the implantation. After tissue
processing, 5-┬Ám sections were prepared and stained by hematoxylin and eosin (H&E) stain. The sections were
studied, and the volume fraction of the newly formed bone was assessed by
Kruskal-Wallis test at a significance level of 0.05.

Results: In the
experimental groups, new bone formation was detected at the margins of the
defects 10 days after the implantation. With the
progression of the healing process, the newly formed bone covered greater areas
of the defects and developed a more mature structure. In the control group, the
defects were primarily filled with a dense connective tissue with small islands
of new bone. The results of histomorphometric assessments showed that the
volume of the newly formed bone in the experimental groups had a significant statistical
difference with that in the control group (P